Background:
@EN It is well known that ultraviolet light(UVL) may cause skin cancer, decrease immune function and promote skin ageing. It is also known that an increase of chlorofluorocabons as air pollution, which causes the depletion of ozone of the earth
in
the
atmosphere, enables harmful ultraviolet-B(UVB) to reach the surface of the earth more easily. The purpose of this study is to determine the harmful effect of UVB on the skin by observing concurrently the morphological and biochemical changes in
the
UBV
irradiated skin.
@ES Objective:
@EN The animal used was the hairless mouse(Hr+/Kud), which are considered to be the most suitable for a UVB irradiation experiment. The Fluorescent sunlamp(Toshiba FL, 20SE, Toshiba electric Co., Tokyo, Japan) was used as a source of UVB.
@ES Method:
@EN The skin of the back and ear was irradiated by an increasing doses of UVB. In morphological changes, the alteration in keratinocytes and Langerhans cells in cell number and morphology were observed. In biochemical changes, activities of the
superoxide dismutase and catalase, which scavanges reactive oxygen species(O2-and H2O2) produced in the skin by UVB irradiation were assayed.
@ES Result:
@EN Sunburn cells appeared at 60mJ/cm* of UVB and increased in number in proportion to the UVB irradiation with dose dependent pattern. The Langerhans cell decreased innumber in proportion to UVB irradiation in dose dependent manner(half maximum
dose
was 60mJ/cm*), and was not found at 1J/cm*. The morphological changes of the Langerhans cells, such as a loss of dendrites and granulation, were noticed at 60mJ /cm* of UVB irradiation. The enzyme activity of catalase decreased in proportion to
UVB
irradiation. The enzyme activity of SOD was not changed by UVB irradiation, however, it significantly increased at 1000mJ/cm* UVB irradiation.
@ES Conclusion:
@EN This study suggest that UVB irradiation to the skin causes a reduction in the immune function and alters the normal biochemical function of the skin. (Kor J Dermatol 1993 ; 31 (2) : 182-190)
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